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Related Experiment Videos

An error-prone viral DNA ligase.

Brandon J Lamarche1, Alexander K Showalter, Ming-Daw Tsai

  • 1Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA.

Biochemistry
|June 9, 2005
PubMed
Summary
This summary is machine-generated.

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African swine fever virus DNA ligase is highly error-prone, unlike other DNA ligases. This low fidelity may contribute to the genetic variability seen in African swine fever virus isolates.

Area of Science:

  • Virology
  • Molecular Biology
  • Enzymology

Background:

  • African swine fever virus (ASFV) DNA polymerase X (Pol X) is known to be error-prone during DNA repair.
  • The functional significance of Pol X infidelity in ASFV genetic variability is uncertain.
  • Downstream DNA ligase activity is crucial for processing nicks, including those with mismatches.

Purpose of the Study:

  • To investigate the nick ligation capabilities of the ASFV-encoded DNA ligase.
  • To determine the 3' fidelity of ASFV DNA ligase using catalytic parameters.
  • To assess if ASFV DNA ligase's fidelity contributes to ASFV genetic variability.

Main Methods:

  • Enzymatic assays were used to determine the catalytic efficiency of nick sealing.
  • Substrates with all 16 possible matched and mismatched base pairs at the 3' side of a nick were employed.

Related Experiment Videos

  • ASFV DNA ligase and bacteriophage T4 DNA ligase were compared.

    • Main Results:

    • ASFV DNA ligase exhibits the lowest fidelity reported for any DNA ligase.
    • It efficiently ligates 3' C:T mismatched nicks, surpassing Watson-Crick base pairs.
    • ASFV DNA ligase's mismatch specificity suggests an evolutionary adaptation for generating diverse sealed mismatches.

    Conclusions:

    • The low fidelity of ASFV DNA ligase likely contributes to the genetic and antigenic variability of ASFV isolates.
    • This finding provides a molecular mechanism for ASFV's adaptability.
    • Novel assays for active DNA ligase concentration were developed.