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Hyperconcentrated platelets stored in additive solution: aspects on productivity and in vitro quality
J Ringwald1, S Walz, R Zimmermann
1Department for Transfusion Medicine and Haemostaseology, University Hospital of Erlangen, Erlangen, Germany. Juergen.Ringwald@trans.imed.uni-erlangen.de
Vox Sanguinis
|June 9, 2005
Summary
New platelet additive solutions require high-concentration platelet concentrates, known as dry platelets (DPs). Storing these DPs in PAS-IIIM shows promising in vitro quality, suggesting a viable approach for platelet transfusion.
Area of Science:
- Transfusion Medicine
- Hematology
- Biotechnology
Background:
- Emerging platelet (PLT) additive solutions (PASs) enable reduced plasma carryover (<30%) in PLT concentrates.
- This necessitates the collection of apheresis PLT concentrates at significantly higher PLT concentrations, termed dry platelets (DPs).
- The TRIMA apheresis system with software version 4 (TRIMA V4) was utilized to collect DPs for quality assessment.
Purpose of the Study:
- To evaluate the efficacy of the TRIMA V4 system in collecting dry platelets (DPs).
- To assess the in vitro quality of DPs stored in a new modified PAS-III (PAS-IIIM) compared to standard PAS-III and plasma.
- To determine the suitability of PAS-IIIM for storing apheresis-derived platelets with low plasma content.
Main Methods:
- TRIMA V4 was programmed to collect DPs at a target concentration of 5000 x 10^3 PLTs/µL.
- Collected DPs were pooled, split, and diluted to create secondary pools with varying ratios of PAS-IIIM, PAS-III, and plasma.
- In vitro quality testing of PLTs was conducted on days 0, 1, 5, and 7, assessing parameters like PLT activation and yield.
Main Results:
- Thirty-two apheresis runs yielded DPs with a concentration of 4255 ± 914 x 10^3 PLTs/µL in approximately 99 minutes.
- Initial in vitro testing revealed 34-37% of PLTs were already activated at day 0.
- Platelets stored in 70% or 80% PAS-IIIM demonstrated superior in vitro quality compared to those stored in PAS-III.
Conclusions:
- The TRIMA V4 system is capable of collecting dry platelets (DPs), although further optimization for higher concentration is desirable.
- Storing apheresis-derived platelets in PAS-IIIM is a highly promising strategy, even with less than 30% plasma carryover.
- PAS-IIIM represents a significant advancement for maintaining platelet quality in low-plasma transfusion products.