Related Experiment Videos
Lecithin:retinol acyltransferase in ARPE-19
Simon G Trevino1, Steven T Schuschereba, Phillip D Bowman
1Department of Biology, the University of Texas at San Antonio, San Antonio, TX 78249, USA.
Experimental Eye Research
|June 9, 2005
Summary
The ARPE-19 cell line can study visual cycle enzymes like lecithin:retinol acyltransferase (LRAT). These cells show active LRAT function and express the LRAT enzyme, making them suitable for in vitro research.
Area of Science:
- Biochemistry
- Cell Biology
- Ophthalmology
Background:
- The visual cycle is crucial for vision, involving enzymes like lecithin:retinol acyltransferase (LRAT).
- Understanding LRAT function in vitro is vital for studying visual disorders.
Purpose of the Study:
- To evaluate the ARPE-19 cell line as a model for studying visual cycle enzyme function.
- Specifically, to determine if ARPE-19 cells can be used to investigate lecithin:retinol acyltransferase (LRAT) activity.
Main Methods:
- ARPE-19 cells were incubated with exogenous retinol.
- LRAT activity was measured in cell membrane proteins.
- LRAT activity was assessed using an inhibitor (retinyl bromoacetate).
- Gene expression and protein levels of LRAT were analyzed via microarray and Western blot.
Main Results:
- ARPE-19 cells accumulated all-trans retinol and all-trans retinyl ester after retinol incubation.
- Membrane proteins from ARPE-19 cells demonstrated LRAT activity.
- This activity was inhibited by retinyl bromoacetate.
- ARPE-19 cells expressed both LRAT transcript and protein.
Conclusions:
- The ARPE-19 cell line possesses active lecithin:retinol acyltransferase (LRAT) enzyme.
- ARPE-19 cells are a suitable in vitro model for studying visual cycle enzymes.
- This cell line can advance research into the biochemistry of vision.