Related Experiment Video
Updated: Aug 17, 2026

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
Published on: May 12, 2023
Characterization of the polymorphic repeat sequence within the rDNA IGS of Cannabis sativa
Hsing-Mei Hsieh1, Chia-Ling Liu, Li-Chin Tsai
1Department of Forensic Science, Central Police University, 56 Shu-Jen Road, Kwei-San, Taoyuan 33334, Taiwan ROC.
Abstract:
The rDNA intergenic spacer (IGS) structure of Cannabis sativa contains six variable repeat motifs within a locus spanning 1387 base pairs. The degree of variation of the first three motifs was examined using 77 samples from cannabis samples. The samples originated from five seizures in Taiwan and seed stocks from six different countries. The results showed that there were four types of sequences producing PCR products at either 255, 260, 264 or 265 base pairs. The data obtained indicates that this region of rDNA IGS exhibits a degree of polymorphism that while insufficient by itself can be added to a multiplex with other cannabis STR loci.
Related Concept Videos
Position-effect Variegation
Cis-regulatory Sequences
Cis-regulatory Sequences
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Eukaryotic RNA Polymerases
All three eukaryotic RNAPs require specific transcription factors, of which the...

