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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

Nanog expression in mouse germ cell development.

Shinpei Yamaguchi¹, Hironobu Kimura, Masako Tada

¹Laboratory of Stem Cell Engineering, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Shogoin, Sakyo-ku, Japan.

Gene Expression Patterns : GEP

|June 9, 2005

Summary

Nanog is crucial for maintaining pluripotency in embryonic stem cells. This study reveals Nanog expression in developing germ cells, highlighting its role in their proliferation and nuclear reprogramming during mammalian development.

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Area of Science:

Developmental Biology
Stem Cell Biology
Reproductive Biology

Background:

Nanog is a key transcription factor maintaining pluripotency in embryonic stem cells.
Its role in germ cell development, particularly temporal and spatial expression, remains largely unknown.
Nanog is known to be highly expressed in primordial germ cells (PGCs) of mouse embryos.

Purpose of the Study:

To investigate the temporal and spatial expression pattern of Nanog in developing germ cells.
To elucidate the function of Nanog during germ cell development and nuclear reprogramming.
To understand sex-specific differences in Nanog expression during germ cell differentiation.

Main Methods:

Immunohistochemistry on whole embryos and cryosections using anti-NANOG and anti-STELLA/PGC7 antibodies.
Analysis of Nanog expression in migrating and colonizing PGCs across different embryonic stages (E7.25-E16.5).
Correlation of Nanog expression with germ cell proliferation, mitotic arrest, and synaptonemal complex formation.

Main Results:

Nanog expression is initially repressed in PGCs, becoming detectable during migration and colonization of genital ridges.
Sex-specific differences in Nanog expression emerge in later embryonic stages.
Nanog expression decreases significantly during mitotic arrest in male PGCs and becomes undetectable in PGCs forming synaptonemal complexes in females.
No Nanog expression is detected in adult germ cells.

Conclusions:

Nanog is expressed in proliferating germ cells undergoing nuclear reprogramming.
The study provides insights into the dynamic role of Nanog during mammalian germ cell development.
Nanog expression is transient and linked to specific stages of germ cell proliferation and differentiation.