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Published on: April 15, 2019
[Studies on the germination of Bacillus anthracoides spores induced by nutrient germinant]
Li-jun Zhang1, Hong-yan Xiong, Yao Zhang
1Department of Military Epidemiology, Academy of Preventive Medicine, The Third Military Medical University, Chongqing 400038, China.
Objective:
To explore the germination effects of Bacillus anthracoides spores germinant to nutrient germinant.
Methods:
Heat factors and nutrient germinant were used to stimulate the Bacillus anthracoides spores and to germinate. Ultraviolet spectrophotometer was used to measured the A value of spore solution in the wavelength of 600 nm. Accrding to the A value, the germination rates in different condition. Transmission electron microscope was used to observe the ultrastructure changes of spores.
Results:
The rate of germination effects were 68.0% under 6 mmol/L inosine at 37 degrees C, pH 7.9; 74.5% under 70 mmol/L L-alanine at 30 degrees C, pH 8.9; and 85.6% under 6 mmol/L inosine and 70 mmol/L L-alanine at 37 degrees C, pH 8.2. Under transmission electron microscope, the germinated spores' coat and cortex were brokendown and degraded with its core completely exposed.
Conclusion:
Under suitable environment, the nutrient germinant with inosine and L-alanine might be helpful for germinating the bacillus anthracoides spores.
