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[Sequence analysis of Echovirus type 30 isolated from an aseptic meningitis outbreak in northern Jiangsu province in

Ya-nan Zhao1, Qing-wu Jiang, Ren-jie Jiang

  • 1Key Laboratory of Public Health Safety, Ministry of Education, School of Public Health, Fudan University, Shanghai 200032, China.

Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi
|June 9, 2005
PubMed
Abstract

Insights

Sequencing echovirus type 30 strains from a 2003 aseptic meningitis outbreak in Jiangsu revealed genetic similarities to earlier strains. This analysis helps understand enterovirus evolution and track outbreaks.

Area of Science:

  • Virology
  • Molecular Biology
  • Epidemiology

Context:

  • Aseptic meningitis outbreaks pose public health challenges.
  • Enteroviruses are a common cause of viral meningitis.
  • Understanding viral genetic diversity is crucial for outbreak response.

Purpose:

  • To determine the partial sequence of echovirus type 30 strains from a 2003 aseptic meningitis outbreak in Jiangsu province.
  • To compare these strains with other international echovirus type 30 isolates.
  • To elucidate the genetic characteristics and evolutionary trends of the outbreak strains.

Summary:

  • Enterovirus RNA was amplified and sequenced using specific primers.
  • Phylogenetic analysis using PHYLIP software and BLAST comparisons were performed.
  • Sequencing identified the outbreak strains as echovirus type 30, closely related to strains from 1999-2000, suggesting distinct evolutionary patterns.

Impact:

  • The 3' half of the VP1 gene sequence is a reliable marker for rapid enterovirus serotype identification.
  • This study provides insights into the genetic relatedness and evolutionary trajectory of echovirus type 30.
  • Findings contribute to better surveillance and control strategies for enteroviral meningitis outbreaks.

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