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Updated: Aug 17, 2026
The Hypoxic Ischemic Encephalopathy Model of Perinatal Ischemia
Published on: November 19, 2008
Hyperoxia with 100% oxygen following hypoxia-ischemia increases brain damage in newborn rats
Roberto Shimabuku1, Arturo Ota, Sonia Pereyra
1Instituto Especializado de Salud del Niño, Lima, Peru. shimabuku@viabcp.com
Objective:
To describe the effect of reoxygenation with 100% O2 as compared to the effect of room air in newborn rat brains after asphyxia.
Methods:
Experimental asphyxia (carotid artery ligation followed by hypoxic exposure with 8% O2 for 2 h) was performed on 7-day-old rats. After hypoxia-ischemia the rats were reoxygenated with either 100% O2 (hyperoxia group) or 21% O2 (room air group) for 24 h and then returned to the dam. The rats were killed 1 week after the experiment to study the cerebral cortex and hippocampus.
Results:
Rats reoxygenated with 100% O2 post-asphyxia showed more frequency of cortical damage (10 of 24 rats) than those reoxy genated with room air (3 of 24 rats) (chi2 test, p = 0.02).
Conclusion:
We consider that hyperoxia with 100% oxygen after hypoxia-ischemia can cause more damage in the cerebral cortex than room air in newborn rats.
