Related Experiment Video
Updated: Aug 17, 2026

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Published on: December 1, 2018
Geometric determination of saturation in fluorescence spectroscopy
1Ente per le Luove Tecnologie, l'Energia e l'Ambiente, Centro Ricerche Casaccia, Via Anguillarese 301, Santa Maria di Galeria, Rome 00060, Italy. michele.marrocco@casaccia.enea.it
Abstract:
In laser spectroscopy, saturation of atomic or molecular transitions cannot be ignored, even at modest laser intensities. The saturation status is customarily diagnosed from measurements of saturation curves describing the dependence of spectroscopic signals on laser intensity. I propose an alternative method that relies on a geometric comparison of the spatial laser profile with images of the spectroscopic quantity under investigation. A single image can be used to determine the saturation status and its associated saturation laser intensity.
More Related Videos
Related Concept Videos
Fluorescence and Phosphorescence: Instrumentation
Spectrophotometry: Introduction
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Flame Photometry: Lab
UV–Vis Spectrometers
Super-resolution Fluorescence Microscopy
Atomic Fluorescence Spectroscopy

