Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparative genomics on Fgf11 orthologs.

Yuriko Katoh1, Masaru Katoh

  • 1M&M Medical BioInformatics, Hongo 113-0033, Japan.

Oncology Reports
|June 10, 2005
PubMed
Summary

This study characterizes the Fgf11 gene across species, revealing conserved elements and expression patterns in human tissues. It provides the first report on the dog Fgf11 gene and comparative genomics of Fgf11 orthologs.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibody-drug conjugates targeting the cadherin, claudin and nectin families of adhesion molecules.

Frontiers in molecular medicine·2025
Same author

Claudin 1, 4, 6 and 18 isoform 2 as targets for the treatment of cancer (Review).

International journal of molecular medicine·2024
Same author

Grand Challenges in Molecular Medicine for Disease Prevention and Treatment Through Cyclical Innovation.

Frontiers in molecular medicine·2024
Same author

Monoclonal antibodies that target fibroblast growth factor receptor 2 isoform b and Claudin-18 isoform 2 splicing variants in gastric cancer and other solid tumours.

Clinical and translational medicine·2024
Same author

FGFR-targeted therapeutics: clinical activity, mechanisms of resistance and new directions.

Nature reviews. Clinical oncology·2024
Same author

Activated FGFR2 signalling as a biomarker for selection of intrahepatic cholangiocarcinoma patients candidate to FGFR targeted therapies.

Scientific reports·2024

Area of Science:

  • Genomics
  • Comparative Biology
  • Molecular Biology

Background:

  • The Fibroblast Growth Factor (FGF) family comprises 22 genes in humans and rodents, with FGF19 being the human ortholog of rodent FGF15.
  • Existing RefSeq data for mouse and rat Fgf11 primarily cover coding regions, necessitating comprehensive sequence determination.

Purpose of the Study:

  • To determine the complete coding sequences of mouse, rat, and dog Fgf11 orthologs.
  • To conduct comparative genomic analyses of Fgf11 orthologs across different vertebrate species.
  • To investigate the conservation of regulatory elements and identify potential functional differences in Fgf11 genes.

Main Methods:

  • Bioinformatic analysis to determine complete coding sequences of Fgf11 orthologs.
  • Sequence alignment and identity comparisons between human, dog, mouse, rat, and zebrafish Fgf11.
  • Analysis of gene structure, including exons and introns, and promoter regions for conserved binding sites.

Main Results:

  • Complete coding sequences for mouse, rat, and dog Fgf11 were determined, with genes located within specific genome sequences and consisting of five exons.
  • High amino-acid identity was observed between human FGF11 and its orthologs in dog (97.8%), mouse (96.9%), and rat (96.9%), with lower identity in zebrafish (68.9%).
  • Conserved Met205 in rat Fgf11, absence of N-terminal signal peptides in vertebrate Fgf11 orthologs, and species-specific glycosylation sites (present in zebrafish, absent in mammals) were identified. Promoter regions of human and mouse Fgf11 showed conserved transcription factor binding sites.

Conclusions:

  • This study provides the first comprehensive report on the dog Fgf11 gene and detailed comparative genomics of Fgf11 orthologs.
  • Conserved coding sequences, promoter elements, and key amino acid residues suggest functional conservation of FGF11 across mammals.
  • Differential glycosylation patterns indicate potential species-specific regulatory mechanisms or functions of FGF11.

Related Experiment Videos