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Related Experiment Videos

CD43 interferes with T-lymphocyte adhesion.

B Ardman1, M A Sikorski, D E Staunton

  • 1Department of Medicine, New England Medical Center Hospitals, Boston, MA 02111.

Proceedings of the National Academy of Sciences of the United States of America
|June 1, 1992
PubMed
Summary

CD43, a cell surface protein, hinders T-cell adhesion to other cells by interfering with leukocyte function-associated antigen 1 binding. This suggests CD43 regulates T-cell interactions and immune responses.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD43 is a cell-surface sialoglycoprotein found on most leukocytes.
  • Its precise physiological role in cell adhesion remains unclear.

Purpose of the Study:

  • To investigate the role of CD43 in intercellular adhesion.
  • To determine if CD43 influences T-lymphocyte binding to other cells.

Main Methods:

  • Compared human T-lymphocyte adherence to HeLa cells expressing CD43 versus CD43-negative HeLa cells.
  • Utilized antibody-blocking experiments to identify adhesion pathways involved.
  • Assessed the impact of phorbol ester and neuraminidase treatments on cell adhesion.

Main Results:

  • Significantly fewer T lymphocytes adhered to CD43-positive HeLa cells compared to CD43-negative cells.

Related Experiment Videos

  • CD43-mediated inhibition of T-cell adhesion was linked to interference with LFA-1/ICAM-1 interactions.
  • Neuraminidase treatment reduced the anti-adhesion effect of CD43, suggesting a role for its sialic acid residues.

    • Conclusions:

    • CD43 expression on opposing cells can inhibit cell-cell adhesion.
    • CD43 may regulate T-cell adhesion by modulating the LFA-1/ICAM-1 pathway, a key lymphocyte activation-induced adhesion mechanism.