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Related Experiment Videos

Compatibility testing problems associated with bovine thrombin-treated plasma.

R S Shirey1, A B Ciappi, T S Kickler

  • 1Blood Bank, The Johns Hopkins Hospital, Carnegie Building, Room 667, 600 N. Wolfe Street, Baltimore, MD 21287-6667, USA.

Immunohematology
|March 1, 1993
PubMed
Summary
This summary is machine-generated.

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Bovine thrombin used to convert plasma to serum contains antibodies that can cause red blood cell agglutination. This can lead to inaccurate blood compatibility testing results due to heteroagglutinins.

Area of Science:

  • Transfusion Medicine
  • Immunology
  • Clinical Pathology

Background:

  • Plasma conversion to serum is a common step before compatibility testing.
  • Bovine thrombin is frequently used for this conversion process.
  • Potential for unexpected serological reactions exists during blood compatibility assessments.

Purpose of the Study:

  • To investigate the cause of red blood cell agglutination observed after bovine thrombin addition.
  • To determine if bovine thrombin itself is associated with serological incompatibilities.
  • To identify the specific components within bovine thrombin responsible for these reactions.

Main Methods:

  • Investigated agglutination of donor red blood cells after conversion of plasma to serum using bovine thrombin.

Related Experiment Videos

  • Analyzed bovine thrombin for the presence of antibodies.
  • Assessed the effect of varying concentrations of bovine thrombin on hemagglutination.

    • Main Results:

    • Bovine thrombin preparation agglutinated all tested donor red blood cells.
    • Thrombin derived from bovine plasma was found to contain IgG antibodies against human red cells.
    • Excessive concentrations of bovine thrombin (> 50 units/mL) induced hemagglutination and potentially erroneous test outcomes.

    Conclusions:

    • Bovine thrombin contains heteroagglutinins (IgG antibodies) capable of causing pan-agglutination.
    • The use of excess bovine thrombin in pre-transfusion testing can lead to false-positive results.
    • Careful consideration of bovine thrombin's antibody content is necessary for accurate serological testing.