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Related Experiment Videos

Bacterial lipopolysaccharide activates suppressor B lymphocytes.

S Koenig, M K Hoffmann

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1979
    PubMed
    Summary

    Lipopolysaccharide (LPS) activates specific B cells that suppress antibody production. These LPS-induced suppressor B cells inhibit immune responses without involving T cells or macrophages.

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    Area of Science:

    • Immunology
    • Microbiology
    • Cell Biology

    Background:

    • Lipopolysaccharide (LPS), a component of Gram-negative bacteria, significantly influences immune responses.
    • LPS can either enhance or inhibit humoral antibody production depending on experimental context.
    • The precise mechanisms underlying LPS-mediated immune suppression require further elucidation.

    Purpose of the Study:

    • To investigate the cellular pathway through which LPS suppresses antibody production.
    • To identify the specific cell type responsible for LPS-induced immune suppression.
    • To characterize the surface antigen phenotype of LPS-activated suppressor cells.

    Main Methods:

    • In vitro incubation of C57BL/6 spleen cells with varying concentrations of LPS.
    • Inhibition assays using spleen cell transfers to assess suppressor cell activity.
    • Flow cytometry and cell surface antigen phenotyping (Ig, Ia, CR, Ly-B-2, PC1) to identify suppressor cells.

    Main Results:

    • High concentrations of LPS (greater than 10 micrograms/ml) inhibited in vitro antibody production to sheep erythrocytes.
    • Adoptive transfer of LPS-stimulated spleen cells suppressed antibody production in non-LPS-treated recipient spleen cells.
    • A specific B cell population, identified as Ig+, Ia+, CR+, Ly-B-2+, PC1+, was determined to mediate LPS-induced suppression.
    • LPS-induced suppressor B cells were generated independently of suppressor macrophages or T cells.

    Conclusions:

    • LPS induces immune suppression primarily through the activation of a distinct B cell subset.
    • These suppressor B cells are the key mediators of LPS-induced inhibition of antibody production.
    • The generation and function of these suppressor B cells do not rely on the presence or activation of suppressor T cells or macrophages.

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