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[Clonal study on single apoptotic cells in myelodysplastic syndromes]
Xiao Li1, Wei-ming Shen, Quan Pu
1Department of Hematology, the Sixth People's Hospital Affiliated to Jiaotong University, Shanghai 200233, China.
Zhonghua Xue Ye Xue Za Zhi = Zhonghua Xueyexue Zazhi
|June 11, 2005
Summary
In myelodysplastic syndromes (MDS), apoptotic hematopoietic cells are predominantly normal, not clonal, cells. This finding suggests residual normal cells undergo apoptosis in MDS patients.
Area of Science:
- Hematology
- Cell Biology
- Oncology
Background:
- Myelodysplastic syndromes (MDS) are a group of clonal hematopoietic stem cell disorders.
- Understanding the cellular origins of apoptosis in MDS is crucial for disease pathogenesis insights.
Purpose of the Study:
- To determine the clonal origin of apoptotic hematopoietic cells in patients with myelodysplastic syndromes (MDS).
Main Methods:
- Simultaneous in situ end labelling (ISEL) and fluorescence in situ hybridization (FISH) on single cells.
- Flow cytometry (FCM) sorting of apoptotic and non-apoptotic cell fractions followed by FISH analysis.
- Karyotyping analysis using G- or R-banding.
Main Results:
- Apoptotic cells in MDS showed a significantly lower percentage of abnormal clonal cells (24.0%) compared to all nucleated cells (37.1%).
- Flow cytometry analysis revealed a higher proportion of normal karyotype cells undergoing apoptosis than non-apoptotic cells in 8/9 MDS patients.
- Fas monoclonal antibody-induced apoptosis did not alter the karyotype of cells.
Conclusions:
- The majority of apoptotic hematopoietic cells in myelodysplastic syndromes are residual normal cells.
- This suggests that normal hematopoiesis is preferentially eliminated during the apoptotic process in MDS.