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Related Experiment Videos

Epithelial Ca(2+) channel expression and Ca(2+) uptake in developing zebrafish.

Tien-Chien Pan1, Bo-Kai Liao, Chang-Jen Huang

  • 1Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan 11529, ROC.

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
|June 11, 2005
PubMed
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Researchers identified the epithelial calcium channel (ECaC) in zebrafish, crucial for calcium absorption during development. This study details its expression and function in young zebrafish, highlighting its role in maintaining calcium homeostasis.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Comparative Physiology

Background:

  • Calcium (Ca2+) uptake is vital for vertebrate development and homeostasis.
  • The epithelial calcium channel (ECaC) is a key player in Ca2+ absorption across various species.
  • Understanding ECaC function in developing fish is essential for aquatic physiology research.

Purpose of the Study:

  • To investigate the role of the epithelial calcium channel (ECaC) in calcium uptake in developing zebrafish (Danio rerio).
  • To clone and characterize the zebrafish ECaC (zECaC) and analyze its expression patterns.
  • To correlate zECaC expression with Ca2+ influx during zebrafish embryonic development.

Main Methods:

  • Cloning and sequencing of full-length cDNA for zebrafish ECaC (zECaC) using rapid amplification of cDNA ends.

Related Experiment Videos

  • Analysis of zECaC gene expression in various zebrafish tissues and developmental stages (24-96 hpf).
  • Measurement of whole-body Ca2+ influx and content in zebrafish embryos under different Ca2+ concentrations.
  • Colocalization studies of zECaC mRNA with Na+-K+-ATPase alpha-subunit to identify expressing cell types.

    • Main Results:

    • Full-length zECaC cDNA (2,578 bp) was cloned, encoding a 709-amino acid protein with high homology to other vertebrate ECaCs.
    • zECaC expression was detected in all examined tissues, starting in the yolk sac skin at 24 hpf and later in gill filaments.
    • Ca2+ influx and content increased in correlation with zECaC expression, particularly from 36-72 hpf.
    • Low-Ca2+ conditions upregulated both Ca2+ influx and zECaC expression in gills and skin.
    • zECaC mRNA was found in a subset of mitochondria-rich cells in the gills.

    Conclusions:

    • The identified zECaC plays a significant role in calcium absorption in developing zebrafish.
    • zECaC expression patterns and Ca2+ influx dynamics are closely linked during embryonic development.
    • Environmental calcium levels influence zECaC expression and overall calcium uptake in zebrafish.