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Related Experiment Videos

ACE2, CBK1, and BUD4 in budding and cell separation.

Warren P Voth1, Aileen E Olsen, Mohammed Sbia

  • 1Department of Pathology, University of Utah, 30 North 1900 East, Salt Lake City, UT 84132-2501, USA.

Eukaryotic Cell
|June 11, 2005
PubMed
Summary

The RAM network and Ace2 transcription factor regulate cell growth and division in yeast. This study reveals Ace2 acts downstream of the RAM network, impacting cell separation and morphology.

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Area of Science:

  • Cell Biology
  • Molecular Genetics
  • Yeast Biology

Background:

  • Mutations in RAM network genes (CBK1, MOB2, KIC1, HYM1, TAO3) disrupt yeast cell polarity and morphology.
  • These defects overlap with phenotypes observed in Ace2 transcription factor mutants, including altered colony morphology and cell separation issues.
  • Ace2 and its paralog Swi5 are key regulators of gene expression involved in cell cycle and morphogenesis.

Purpose of the Study:

  • To elucidate the relationship between the RAM network and the Ace2 transcription factor.
  • To investigate the roles of Ace2, Cbk1, and Bud4 in yeast bud site selection, polarized growth, and cell separation.
  • To characterize the genetic interactions and regulatory pathways governing these cellular processes.

Main Methods:

  • Genetic analysis of RAM network and Ace2/Swi5 mutants in yeast (Saccharomyces cerevisiae).

Related Experiment Videos

  • Plasmid-based complementation assays to assess gene function and pathway relationships.
  • Timed sedimentation assays to quantify cytokinesis defects and analyze cell shape.
  • Analysis of gene expression for Ace2 and Swi5 target genes.

    • Main Results:

    • Ace2 functions downstream of the RAM network, with reduced Ace2 activity contributing to RAM mutant phenotypes.
    • A novel timed sedimentation assay effectively quantifies cytokinesis defects and budding patterns.
    • Ace2 and Cbk1 possess both shared transcriptional roles (e.g., CTS1 regulation) and independent functions in cell polarization and separation.
    • Genetic interactions between bud4, ace2, and cbk1 mutants reveal complex regulatory networks.

    Conclusions:

    • The RAM network and Ace2 pathway are crucial for proper yeast cell division and polarity.
    • Ace2 is a key downstream effector of the RAM network, mediating phenotypes related to cell separation and morphology.
    • Distinct and overlapping functions of Ace2, Cbk1, and Bud4 highlight the intricate regulation of yeast morphogenesis.