Related Experiment Video
Updated: Aug 17, 2026
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Published on: February 1, 2019
N4,N9-dioleoyl spermine is a novel nonviral lipopolyamine vector for plasmid DNA formulation
Osama A A Ahmed1, Noppadon Adjimatera, Charareh Pourzand
1Department of Pharmacy and Pharmacology, University of Bath, Bath, BA2 7AY, United Kingdom.
Purpose:
To study the effect of synthesized N4,N9-dioleoyl spermine on DNA condensation and then measure its transfection efficiency in cell culture.
Methods:
The lipopolyamine was synthesized from the naturally occurring polyamine spermine. The ability of this novel compound to condense DNA was studied using ethidium bromide fluorescence quenching and light scattering assays. Transfection efficiency was studied in primary skin cells (FEK4) and in an immortalized cancer cell line (HtTA), and compared with the commercially available transfection formulations Lipofectin and Lipofectamine.
Results:
The synthesized N4,N9-dioleoyl spermine formula is efficient at condensing calf thymus and circular plasmid DNA and effectively transfects both primary skin cells and cancer cell lines at low charge ratios of (+/- ammonium/phosphate) 2.5.
Conclusions:
N4,N9-Dioleoyl spermine condenses DNA and achieves high transfection levels in cultured cells.
