Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Housekeeping genes in cancer: normalization of array data.

Anis H Khimani1, Abner M Mhashilkar, Alvydas Mikulskis

  • 1PerkinElmer Life Sciences, Boston, MA, USA.

Biotechniques
|June 14, 2005
PubMed
Summary

Housekeeping genes, essential for cell stability, unexpectedly changed expression in cancer models. This finding cautions against using them for normalizing gene expression data in cancer research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intraductal papillary mucinous neoplasm-derived pancreatic cancer of the ventral duct in patient with pancreas divisum: a case report.

Journal of surgical case reports·2026
Same author

Nanotechnology-Based Device Reduces Pain and Immediate Opioid Requirements and Facilitates Earlier Discharge From the Hospital Following Total Knee Arthroplasty: A Randomized Placebo-Controlled Trial.

The Journal of arthroplasty·2026
Same author

Relationship of Distance to PCI Center With In-Hospital Mortality After STEMI: Insights From a Rural Regional STEMI Network.

Journal of the Society for Cardiovascular Angiography & Interventions·2026
Same author

Standards, Strategies, and Innovations in Cardiac Point-of-Care Ultrasound Education: A Scoping Review.

Cardiology in review·2026
Same author

Protein Analysis of the Surface of Orthokeratology Lenses.

Case reports in ophthalmology·2025
Same author

Promoting and Supporting Positive Conversations and Knowledge Mobilisation About Organ Donation in NHS Staff: a Hashtag "#" Series of Projects.

Transplant international : official journal of the European Society for Organ Transplantation·2025

Area of Science:

  • Molecular Biology
  • Genomics
  • Cancer Research

Background:

  • Housekeeping genes are assumed to maintain stable expression levels across different cellular conditions.
  • Deviations in housekeeping gene expression can impact the reliability of gene expression analysis.
  • Cancer and gene therapy can introduce cellular variability affecting gene expression.

Purpose of the Study:

  • To investigate variations in housekeeping gene expression in cancer models.
  • To assess the suitability of traditional housekeeping genes for normalization in cancer research.
  • To highlight potential issues in gene expression analysis due to altered housekeeping gene levels.

Main Methods:

  • DNA microarray technology was employed for high-throughput gene expression analysis.

Related Experiment Videos

  • Quantitative PCR (qPCR) was used to validate differential gene expression findings.
  • Prostate cancer and a colorectal cancer gene therapy model system were utilized.

    • Main Results:

    • Differential expression of specific housekeeping genes was observed in both cancer models.
    • Ribosomal protein genes showed altered expression in prostate cancer cells.
    • Beta-actin exhibited differential expression in treated colorectal cancer cells.

    Conclusions:

    • The expression of certain housekeeping genes is not always stable in cancer.
    • Caution is advised when using housekeeping genes for normalization in cancer gene expression studies.
    • Findings underscore the need for careful selection and validation of reference genes in cancer research.