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Related Experiment Videos

Gene expression in the preimplantation embryo: in-vitro developmental changes.

Shunping Wang1, Chad A Cowan, Hiram Chipperfield

  • 1Boston IVF, 40 Second Avenue, Suite 200, Waltham, MA 02451, USA. shunping.wang@bostonivf.com

Reproductive Biomedicine Online
|June 14, 2005
PubMed
Summary

In vitro embryo culture shows minimal gene expression differences compared to in vivo development. However, DNA methyltransferase 1 (DNMT1) gene expression is significantly higher in cultured embryos, impacting assisted reproductive technology safety.

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Area of Science:

  • Developmental Biology
  • Genomics
  • Reproductive Medicine

Background:

  • Early embryo development and implantation mechanisms are complex and not fully understood.
  • In vitro culture effects on embryo development raise safety concerns for assisted reproductive technology (ART).
  • Previous studies reported individual gene expression changes in cultured embryos, but large-scale comparisons are lacking.

Purpose of the Study:

  • To conduct a large-scale gene expression comparison between in vitro cultured and in vivo mouse embryos.
  • To investigate the impact of in vitro culture on gene expression profiles across eight preimplantation stages.
  • To identify specific genes with altered expression patterns due to in vitro culture.

Main Methods:

  • Microarray technology was used to analyze gene expression profiles of over 20,000 genes.

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  • Mouse embryos were analyzed at eight developmental stages: oocytes, zygote, 2-, 4-, 8-cell, compacting embryo, morula, and blastocyst.
  • Gene expression data from in vitro cultured embryos were compared with in vivo counterparts.

    • Main Results:

    • Overall gene expression patterns showed minimal significant differences between in vitro and in vivo embryos across most developmental stages.
    • DNA methyltransferase 1 (DNMT1) exhibited significantly higher expression (P < 0.05) in cultured embryos.
    • Cadherin-11 showed a slightly altered pattern, though not statistically significant; other developmentally important genes had similar expression profiles.

    Conclusions:

    • In vitro culture has a largely similar effect on overall gene expression compared to in vivo development for mouse preimplantation embryos.
    • Elevated DNMT1 expression in cultured embryos warrants further investigation regarding its implications for ART.
    • The study provides a comprehensive gene expression dataset for assessing the impact of in vitro culture on early embryonic development.