Related Experiment Video
Updated: Aug 17, 2026
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
[Detection of CCAAT/enhancer binding protein alpha gene mutations in acute myeloid leukemia]
Jin-mei Zhao1, Hong-wei Wang, Zhi-fang Xu
1Institute of Hematology, 2nd Hospital of Shanxi Medical University, Taiyuan 030001, China.
Objective:
To explore the relationship between CCAAT/enhancer binding protein alpha (C/EBPalpha) gene mutations and the development of acute myeloid leukemia (AML).
Methods:
The whole coding region of C/EBPalpha gene were screened in 48 cases of AML and 11 normal subjects by PCR-single strand conformation polymorphism (PCR-SSCP) and sequencing.
Results:
C/EBPalpha mutations were detected in 5 of 48 AML patients. Four duplications and 1 deletion were confirmed by DNA sequencing. All of those are newly identified mutations.
Conclusions:
Different mutation types of C/EBPalpha gene exist in a small number of patients with AML and might be related to the pathogenesis of some leukemias.
