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Updated: Aug 7, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
Rapid assignment of protein side chain resonances using projection-reconstruction of (4,3)D HC(CCO)NH and
Ling Jiang1, Brian E Coggins, Pei Zhou
1Department of Biochemistry, Duke University Medical Center, 242 Nanaline Duke Building, Research Drive, Durham, NC 27710, USA.
Abstract:
The reconstruction of higher-dimensional NMR spectra from projections can provide significant savings in instrument time. Here, we demonstrate its application to the (4,3)D HC(CCO)NH and intra-HC(C)NH experiments. The latter experiment contains a novel intra-residue filter element, which selectively correlates the side chain resonances with the corresponding intra-residue amide resonances. Compared to the conventional HC(C)NH experiment, the intra-HC(C)NH experiment reduces the spectral complexity and thus the minimum number of projections required for artifact-free reconstruction by half. The use of the projection-reconstruction technique allows rapid data collection and unambiguous assignment of aliphatic side chain nuclei at high resolution.
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