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Invasiveness in in vitro and clinical evaluation of meningiomas
1Laboratory for Histology, University of Gent, Belgium.
Abstract:
Fragments of freshly isolated human meningiomas were cultured in vitro to form cell monolayers. These monolayers were confronted with embryonic chick heart fragments in vitro for 1, 2, 4, and 7 days. Microscopically, three different histological patterns were observed. Type I included necrotized meningial cells; type II presented surviving meningial cells; type III included meningial cells that had invaded the host tissue. The clinical analysis included the histopathological diagnosis, the macroscopic situation at surgical intervention, and the follow-up with or without recurrence. Correlation between these clinical parameters and the in vitro results demonstrated that type III confrontations correlated with macroscopic infiltration in the brain parenchyma and tumor recurrence. Invasiveness in vitro was seen in two anaplastic and two transitional meningiomas.
Insights
Human meningioma cells cultured in vitro showed invasiveness. Type III cells invaded host tissue, correlating with brain infiltration and tumor recurrence in patients.
Area of Science:
- Neurosurgery
- Oncology
- Cell Biology
Background:
- Meningiomas are tumors arising from the meninges.
- Understanding meningioma invasiveness is crucial for predicting recurrence and patient outcomes.
Purpose of the Study:
- To investigate the in vitro invasiveness of human meningioma cells.
- To correlate in vitro invasiveness with clinical parameters such as infiltration and recurrence.
Main Methods:
- Human meningioma fragments were cultured in vitro to form cell monolayers.
- Meningioma cell monolayers were co-cultured with embryonic chick heart fragments for up to 7 days.
- Histological patterns of cell interaction were analyzed, categorizing invasiveness into three types.
Main Results:
- Three distinct in vitro invasion patterns were observed: necrotized cells (Type I), surviving cells (Type II), and invasive cells (Type III).
- Type III in vitro invasiveness was significantly correlated with macroscopic brain parenchymal infiltration during surgery.
- Type III invasiveness was also associated with a higher rate of tumor recurrence.
Conclusions:
- In vitro invasiveness of meningioma cells can be modeled using co-culture systems.
- The in vitro invasion pattern, particularly Type III, serves as a potential predictor for aggressive tumor behavior, including infiltration and recurrence.