Related Experiment Video
Updated: Aug 17, 2026

Exploring Independent Effects of Follicle-Stimulating Hormone In Vivo in a Mouse Model
Published on: August 11, 2023
Ovarian response and follicular development for single-dose and multiple-dose protocols for gonadotropin-releasing
Tsung-Hsien Lee1, Ming-Yih Wu, Hsin-Fu Chen
1Department of Obstetrics and Gynecology, College of Medicine and National Taiwan University Hospital, National Taiwan University, Taipei, Taiwan.
Objective:
To determine the efficiency of a single-dose and a multiple-dose protocol for GnRH antagonist administration.
Design:
Randomized clinical trial.
Setting:
University hospital, tertiary medical center.
Patient(S):
Sixty-one patients undergoing controlled ovarian stimulation (COS) and IVF/ICSI.
Intervention(S):
COS with either a multiple-dose (MD) or a single-dose (SD) protocol for GnRH antagonist (cetrorelix) administration, or with a long protocol (LP) for GnRH agonist (buserelin) administration, followed by oocyte retrieval, IVF/ICSI, and embryo transfer.
Main Outcome Measure(S):
Follicular development and serum levels of E2 and LH.
Result(S):
The SD protocol for cetrorelix was associated with a more reduced level of follicular development, lower levels of serum estradiol on the day of HCG administration, and a more reduced number of zygotes than the LP for buserelin. The pregnancy and implantation rates did not differ significantly for the three study groups.
Conclusion(S):
The MD and SD GnRH antagonist protocols were effective for preventing LH surge and appear to elicit an equivalent pregnancy rate to that corresponding to a LP GnRH agonist. In terms of follicular development, the SD protocol requires further modification, including flexible scheduling or possibly a small reduction of the dosage of the administered cetrorelix.
Related Concept Videos
Bioavailability Study Design: Single Versus Multiple Dose Studies
Ovarian Cycle
Determination of Multiple Dosing Parameters: Loading and Maintenance Doses
Dose-Response Relationship: Overview
Drug Accumulation During Multiple Dosing: Repetitive IV Injections
Dosage Regimen: Fixed Dose
Fixed-dose regimens can be used for various routes of administration, including intravenous (IV) injections and oral medications. For IV administration, a predetermined amount of the drug is...