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Updated: Aug 17, 2026
Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
Published on: April 29, 2015
Expression and regulation of CCR1 in peritoneal macrophages from women with and without endometriosis
Fritz Wieser1, Songül Dogan, Karin Klingel
1Center for Reproductive Sciences, Department of Obstetrics and Gynecology, University of California, San Francisco, California, USA.
Abstract:
CCR1 is a CC chemokine receptor with high affinity for RANTES (regulated upon activation, normal T cells expressed and secreted). CCR1 protein and mRNA concentrations in native peritoneal cells were twofold greater, in cultured peritoneal cells threefold greater, in patients with endometriosis compared to patients without endometriosis, as determined by Western blotting fluorescence activated cell sorting analysis, reverse transcription-polymerase chain reaction, and in situ hybridization.