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Preparation of B lymphocyte-specific alloantisera by skin implant immunization of cattle

J T Mackie1, M J Stear

  • 1Department of Immunology, John Curtin School of Medical Research, Australian National University, Canberra, A.C.T.

Veterinary Immunology and Immunopathology
|January 15, 1992
PubMed

B lymphocyte-specific antisera were prepared by immunizing cattle on either one or two occasions with a subcutaneous implant of allogeneic skin and subsequently absorbing the antisera with platelets. After absorption 15/26 antisera displayed B lymphocyte-specific activity. Titres against B-enriched cells were 8-64 while residual titres against B-depleted cells were 1-8. In comparison, 3-6 immunizations with allogeneic leucocytes produced antisera of similar peak cytotoxic titres against donor PBL, and after platelet absorption 8/15 antisera displayed B lymphocyte-specific activity. Titres against B-enriched cells were 8-64 while residual titres against B-depleted cells were 2-8. The skin implant method was less time-consuming than the leucocyte immunization method.

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