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Updated: Aug 17, 2026
Chromosome Preparation From Cultured Cells
Published on: January 28, 2014
[Molecular cytogenetic analysis for a familial complex chromosomal rearrangement]
Wei-ping Qian1, Yue-qiu Tan, Wai-mui Tjia
1Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, Hunan, 410078, PR China.
Objective:
To determine a complex chromosomal rearrangement by advanced molecular cytogenetic techniques and analyze its clinical effect.
Methods:
A complex chromosomal rearrangement (CCR) involved in chromosomes 5, 16 and 20 in a 29-year-old male carrier was determined by chromosomal microdissection and multicolor fluorescence in situ hybridization (M-FISH), and family degree investigation was further performed.
Results:
The karyotype of the case was a complex chromosomal translocation among chromosomes 5, 20 and 16, and accompanied with a band of chromosome 20 inserted into chromosome 5. His mother and sister both had the same abnormal karyotype by familial investigation.
Conclusion:
The combined use of M-FISH and chromosome microdissection is a powerful tool to determine CCR. The complex chromosomal rearrangement could be transmitted stably in the family, but still the carriers could give birth to a healthy baby by chance.
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