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[Role of microvessel density on human middle ear cholesteatoma]
1Department of Otorhinolaryngology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China. linrenyu@sohu.com
Objective:
To explore the role of microvessel density in acquired middle ear cholesteatoma, to analyze it playing the possible role in proliferative ability of cholesteatoma epidermis.
Methods:
The specimens from 15 cases acquired middle ear cholesteatoma patients having typical perforation in the posterior fold (the adjacent skin around perforation and the normal deep external ear skin) were examined by immunohistochemical ABC method. The microvessel density was analyzed by computer, and was compared with 20 cases non-cholesteatoma otitis media's the adjacent skin around perforation.
Results:
Counts of microvessel and relative area of all microvessels per image in the adjacent skin around perforation were 14.395 +/- 2.000 and (9.927 +/- 2.600)%, respectively. These were significantly higher than the normal deep external ear skin's with the average of 6.218 +/- 0.949 and (5.076 +/- 0.807)% in 15 cases acquired middle ear cholesteatoma patients (P < 0.001). The adjacent skin around perforation of cholesteatoma was also significantly higher than non-cholesteatoma's with the average of 6.163 +/- 1.051 and (5.785 +/- 1.428)% (P < 0.001).
Conclusions:
The adjacent skin around perforation in human middle ear cholesteatoma had a relatively hyperproliferative ability.
Insights
Microvessel density is significantly elevated in the skin surrounding perforations in acquired middle ear cholesteatoma, indicating a hyperproliferative capacity. This finding suggests a role for microvessels in the growth of cholesteatoma epidermis.
Area of Science:
- Otolaryngology
- Histopathology
- Angiogenesis research
Context:
- Acquired middle ear cholesteatoma is a destructive condition.
- The proliferative capacity of cholesteatoma epidermis is not fully understood.
- Microvessel density is a marker of tissue vascularity and potential proliferation.
Purpose:
- To investigate the microvessel density in acquired middle ear cholesteatoma.
- To determine if microvessel density correlates with the proliferative ability of cholesteatoma epidermis.
Summary:
- Immunohistochemical analysis of 15 acquired middle ear cholesteatoma specimens and 20 non-cholesteatoma otitis media controls was performed.
- Microvessel density and relative microvessel area were significantly higher in the skin adjacent to cholesteatoma perforations compared to normal ear skin and non-cholesteatoma controls.
- These elevated levels suggest increased angiogenesis and a hyperproliferative state in the affected skin.
Impact:
- The findings suggest that increased microvessel density contributes to the hyperproliferative nature of the epidermis in acquired middle ear cholesteatoma.
- This research may inform future therapeutic strategies targeting angiogenesis in cholesteatoma management.
