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[Biological effects of nano-hydroxyapatite/polyamide 66 on the osteoblast]
Ling Ye1, Qin Su, Xue-Dong Zhou
1Key Lab. of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Objective:
To evaluate the biological effects of nano-hydroxyapatite/polyamide 66(nHA-PA66) on the growth and activity of osteoblast.
Methods:
MTT assay was used to determine the growth of osteoblast, enzymatic measure was used to determine the activity of ALP and quantitative RT-PCR (QRT-PCR) to evaluate the changes of osteoclacin mRNA expression in osteoblasts treated by DMEM eluate of nHA-PA66.
Results:
Osteoblasts of different test groups demonstrated relative proliferation rate ranging from 98% - 106% without dose-dependent effect. The ALP activity and osteocalcin mRNA expression were similar in test and control groups (P > 0.05).
Conclusion:
nHA-PA66 has no negative effects on the osteoblast and its osteoblast-compatibility is proved.
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