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Published on: July 7, 2017
[Study of IL-8 overexpression in A549 cells induced by crocidolite fibers]
Xin-chao Wang1, Yu-bao Xu, Yi-ming Wu
1School of Public Health, Zhengzhou University, Zhengzhou 450052, China.
Objective:
This study was to investigate the effect of crocidolite fibers on IL-8 protein and mRNA levels in A549 cells cultured supernatant and the role of tyrosine kinase inhibitor PD98059 on IL-8 expression.
Methods:
Real Time PCR and ELISA were employed to detect A549 cellular IL-8 mRNA and supernatant IL-8 protein, respectively.
Results:
After incubating cells with 100 microg/ml of crocidolite, IL-8 protein releasing in supernatant was significantly increased and IL-8 mRNA was also higher than that of control, the differences were statistically significant (P < 0.05), however both IL-8 protein and mRNA was abrogated by pretreating cells with tyrosine kinase inhibitor PD98059.
Conclusion:
IL-8 was shown as overexpression and could be decreased by PD98059. This indicated that MAPK signal pathway is involved in the process IL-8 expression.
