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Updated: Aug 17, 2026
Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Published on: August 6, 2011
[Application with single-stranded probes in randomized terminal linker-dependent PCR]
Chao-long Rao1, Qing Wu, Zheng-chang Heng
1School of Public Health, Sichuan University, Chengdu 610041, China.
Objective:
Preparing single-stranded (ss) probes in place of double-stranded (ds) probes to improve the hybridization efficiency and specificity of randomized terminal linker-dependent PCR (RDPCR).
Methods:
Using asymmetric PCR and single-primer PCR to prepare ss probes of extron 7 of p53 gene in rat, then comparing the results hybridized with ss probes and ds probes.
Results:
Preparation of ss probes by asymmetric PCR and single-primer PCR gets success. Hybridization results showed that the ss probes could get better signals and less noise than ds probes.
Conclusion:
In comparison with ds probes, the application of ss probes can increase the hybridization sensitivity and specificity of RDPCR.
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