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Published on: December 14, 2019
[Study of the genetic stability of expression plasmid vector pMG36e in host bacteria]
Chuan Wang1, Chao-wu Zhang, Jiang Du
1Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Objective:
To study the genetic stability of expression plasmid vector pMG36e in Escherichia coli JM109 and Lactococcus lactis MG1363/36e and observe the effect of erythomycin for its stability.
Methods:
We used classical method to determine the genetic stable rates of pMG36e in E. coli JM109 and Lactococcus lactis MG1363/36e with or without erythomycin selective pressure.
Results:
When E. coli JM109 were continuously incubated to 120 generations, its pMG36e genetic stable rates were 100% and 96% respectively with or without erythomycin selective pressure. When Lactococcus lactis MG1363/36e were continuously incubated to 100 generations, its pMG36e genetic stable rates were the same 98%, with or without erythomycin selective pressure.
Conclusion:
pMG36e had good genetic stability in this two hosts. Erythromycin selective pressure had no significant effect for the genetic stability of pMG36e.
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