Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural insights into translational fidelity.

James M Ogle1, V Ramakrishnan

  • 1Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom. james.ogle@cantab.net

Annual Review of Biochemistry
|June 15, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular architecture and diversity of StopGo/2A translational recoding.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Purification and characterization of recombinant human translation initiation factor eIF3.

Protein science : a publication of the Protein Society·2025
Same author

Efficient circular RNA synthesis for potent rolling circle translation.

Nature biomedical engineering·2024
Same author

Semorinemab Pharmacokinetics and The Effect on Plasma Total Tau Pharmacodynamics in Clinical Studies.

The journal of prevention of Alzheimer's disease·2024
Same author

Human tumor suppressor protein Pdcd4 binds at the mRNA entry channel in the 40S small ribosomal subunit.

Nature communications·2024
Same author

Genetic predisposition of BDNF (rs6265) gene is susceptible to Schizophrenia: A prospective study and updated meta-analysis.

Neurologia·2024

Recent studies reveal the structural basis of transfer RNA (tRNA) selection during protein synthesis. This mechanism involves ribosome-induced conformational changes that enhance the accuracy of translation and GTP hydrolysis by EF-Tu.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • Protein synthesis accuracy is crucial for cellular function.
  • Decades of research have sought to elucidate the mechanisms of translational fidelity.
  • Recent advances in biochemical and structural data provide new insights.

Purpose of the Study:

  • To outline the structural basis for transfer RNA (tRNA) selection during protein synthesis.
  • To explain how ribosome-induced conformational changes influence GTP hydrolysis by EF-Tu.
  • To connect these mechanisms to observed effects of mutations and antibiotics on translational fidelity.

Main Methods:

  • Analysis of recent biochemical and structural data.
  • Investigation of tRNA selection and codon recognition.

Related Experiment Videos

  • Study of GTP hydrolysis by elongation factor-Tu (EF-Tu).

    • Main Results:

    • Cognate tRNA binding induces ribosome conformational changes that stabilize the transition state for GTP hydrolysis by EF-Tu.
    • The ribosome monitors the first two codon-anticodon positions strictly but the third (wobble) position less stringently.
    • A distorted tRNA is characteristic of the GTP hydrolysis transition state, explaining antibiotic and mutation effects.

    Conclusions:

    • The structural basis for tRNA selection and its role in translational accuracy are becoming clearer.
    • Understanding these mechanisms provides a framework for interpreting effects of genetic mutations and antibiotics.
    • Further research is needed to fully understand GTP hydrolysis activation and the decoding-frameshifting relationship.