Asphyxia and depolarization increase adenosine levels in perilymph

Extracellular adenosine has been suggested as a modulator of cochlear function. To date the release of adenosine into the extracellular spaces of the cochlea has not been demonstrated. Therefore, experiments were designed to examine whether adenosine release into perilymph could be detected in response to depolarization by high potassium concentrations or in response to asphyxia. For this purpose, the perilymph compartment of guinea pigs was perfused with an artificial perilymph and the effluent assayed for ATP, ADP, AMP and adenosine. Results indicate that potassium induced a slight, significant increase and asphyxia induced a very large, significant increase in adenosine levels in perilymph effluent. No changes in the levels of the other compounds were measured. It is concluded that depolarization and asphyxia can induce the release of adenosine into perilymph.