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Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection
Published on: June 12, 2014
Entamoeba histolytica: identification of EhGPCR-1, a novel putative G protein-coupled receptor that binds to EhRabB
Karina Picazarri1, Juan Pedro Luna-Arias, Eduardo Carrillo
1Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del IPN, A.P. 14-740, Mexico, D.F. 07000, Mexico.
Abstract:
EhRabB is an Entamoeba histolytica protein involved in phagocytosis. However, proteins that regulate the EhRabB activity are unknown. Here, we report the identification of a putative G protein-coupled receptor of E. histolytica (EhGPCR-1) that binds to EhRabB. By two-hybrid screening, we found a 372-bp cDNA fragment that encodes the C-terminus of EhGPCR-1. The cloning and sequence of the full-length cDNA revealed that it predicts a polypeptide with two tyrosine-based sorting signals for endocytosis and seven transmembranal domains. These results suggest that EhGPCR-1 could be a GPCR involved in phagocytosis. EhGPCR-1 could be a member of the Rhodopsin family, characterized by a short N-terminus without cysteine residues.
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