Related Experiment Video
Updated: Aug 17, 2026
Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds
Published on: October 29, 2015
Improved attachment of natural HCV isolate to Daudi cells upon elimination of immune complexes and close pH control
Josep Quer1, Joan Cos, Pedro Murillo
1Liver Unit, Department of Medicine, Hospital Universitari Vall d'Hebron, Barcelona, Spain. jquer@vhebron.net
Objectives:
To study the viral requirements for attachment, entry and infection using natural isolates of HCV (infectious plasma of a patient infected with HCV from genotype 1b) and a cell line which has previously been shown to support HCV replication (Daudi cells).
Methods:
We studied attachment of HCV to Daudi cells, a human B-cell line. Quantification was done by real-time RT-PCR.
Results:
The best attachment levels were obtained using plasma depleted of immunocomplexes. Results of kinetics of HCV attachment to Daudi cells show that attachment is maximum at pH 7.0, and that it decreased drastically at any other pH studied (5.5, 6.0, 6.5, 7.5 and 8.0).
Conclusions:
Depletion of immunocomplexes and pH control during infection are important parameters to improve attachment of HCV to Daudi cells using plasma as a natural source of virus.
