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Microbial growth in various perfluorocarbon liquids

Lider Celik1, Mine Yücesoy, A Osman Saatci

  • 1Department of Ophthalmology, University of Dokuz Eylül, Izmir, Turkey.

Ophthalmic Surgery, Lasers & Imaging : the Official Journal of the International Society for Imaging in the Eye
|June 17, 2005
PubMed

Insights

Perfluorocarbon liquids, used in eye surgery, do not support microbial growth. This finding indicates they do not increase the risk of endophthalmitis, a serious eye infection.

Area of Science:

  • Ophthalmology
  • Microbiology
  • Biomaterials Science

Background:

  • Perfluorocarbon liquids are utilized in intraocular surgery.
  • Contamination of these liquids with microbes is a potential risk.
  • Endophthalmitis is a severe post-operative complication.

Purpose of the Study:

  • To evaluate the potential of perfluorocarbon liquids to support microbial growth.
  • To assess the risk of microbial contamination in intraocular surgery using these liquids.

Main Methods:

  • Prospective in vitro study.
  • Tested perfluorodecaline, perfluoro-noctane, and perfluorophenanthrene.
  • Inoculated with Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans.
  • Utilized tryptone soy broth, pancreatic digest of casein, and Sabouraud broth.
  • Incubation for up to 10 days.

Main Results:

  • No microbial growth was observed in any tested perfluorocarbon liquids.
  • The tested perfluorocarbon liquids demonstrated no capacity to support microbial proliferation.

Conclusions:

  • Perfluorocarbon liquids do not promote microbial growth.
  • Their use in vitreoretinal surgery does not appear to increase the risk of endophthalmitis.

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