Related Experiment Video

Updated: Aug 17, 2026

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

Novel interaction between nuclear coactivator CBP and the protein inhibitor of activated Stat1 (PIAS1)

Xiaolong Yin1, Dennis R Warner, Emily A Roberts

  • 1University of Louisville Birth Defects Center, Department of Molecular, Cellular and Craniofacial Biology, Louisville, KY 40292, USA.

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
|June 17, 2005
PubMed

Insights

Protein inhibitor of activated Stat1 (PIAS1) binds to cAMP response element binding protein (CREB)-binding protein (CBP). PIAS1 inhibits CBP

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Gene Regulation

Background:

  • cAMP response element binding protein (CREB)-binding protein (CBP) is a key transcriptional coactivator involved in gene regulation.
  • CBP expression is developmentally regulated in murine embryonic orofacial tissues, suggesting a role in development.

Purpose of the Study:

  • To identify novel nuclear factors interacting with CBP in developing embryonic orofacial tissue.
  • To investigate the functional consequences of CBP-interacting proteins on CBP's transcriptional activity.

Main Methods:

  • Yeast two-hybrid screening using the carboxy-terminal region of CBP as bait.
  • Glutathione S-transferase (GST) pull-down assays for in vitro binding confirmation.
  • Coimmunoprecipitation for in vivo interaction validation.
  • Reporter assays to assess effects on CBP-mediated transcription.

Main Results:

  • Protein inhibitor of activated Stat1 (PIAS1) was identified as a novel CBP-binding protein.
  • The interaction between PIAS1 and CBP was confirmed through both in vitro and in vivo methods.
  • PIAS1 was found to inhibit CBP-mediated transcriptional activation, independent of transforming growth factor-beta (TGF-β).

Conclusions:

  • PIAS1 is a novel binding partner for CBP.
  • PIAS1 acts as an inhibitor of CBP-mediated transcription.
  • These findings suggest PIAS1 may regulate embryonic development processes like cell proliferation, migration, and differentiation.

More Related Videos

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions07:03

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Published on: December 23, 2022

Related Concept Videos

The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...