Related Experiment Video

Updated: Aug 17, 2026

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform
06:21

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform

Published on: May 10, 2024

Cytotoxic effects of pemetrexed in gastric cancer cells

Jee Hyun Kim1, Keun-Wook Lee, Yeonjoo Jung

  • 1Department of Internal Medicine, Seoul National University College of Medicine, 28, Yongon-Dong, Chongno-Gu, Seoul.

Cancer Science
|June 17, 2005
PubMed

Insights

Pemetrexed shows significant cytotoxicity against gastric cancer cell lines, often exceeding 5-fluorouracil. Its combination with cisplatin demonstrates additive or synergistic effects, supporting clinical use, though sensitivity is not predicted by key gene expressions.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Pemetrexed is a novel antifolate drug with demonstrated efficacy in various solid tumors.
  • Gastric cancer remains a significant global health challenge, necessitating novel therapeutic strategies.

Purpose of the Study:

  • To evaluate the cytotoxic effects of pemetrexed in gastric cancer cell lines.
  • To investigate the interaction between pemetrexed and cisplatin.
  • To identify potential genetic markers predicting pemetrexed sensitivity.

Main Methods:

  • Cytotoxicity assessed using MTT assay.
  • Drug interactions evaluated via isobologram analysis.
  • Gene expression analysis (protein and mRNA) for thymidylate synthase (TS), folylpoly-gamma-glutamate synthetase (FPGS), and reduced folate carrier (RFC1).

Main Results:

  • Pemetrexed exhibited greater cytotoxicity than 5-fluorouracil across tested gastric cancer cell lines.
  • The combination of pemetrexed and cisplatin displayed additive or synergistic interactions.
  • No significant correlation was found between TS, FPGS, or RFC1 expression levels (protein or mRNA) and pemetrexed sensitivity.

Conclusions:

  • Pemetrexed demonstrates significant activity against gastric cancer cell lines.
  • The combination therapy of pemetrexed and cisplatin warrants further clinical investigation for gastric cancer treatment.
  • Pemetrexed sensitivity in gastric cancer appears to be multifactorial, not predictable by the expression of TS, FPGS, or RFC1 alone.

More Related Videos

Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT10:28

Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT

Published on: January 22, 2018

Related Concept Videos

Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Gastritis II: Pathophysiology01:26

Gastritis II: Pathophysiology

The pathophysiology of gastritis begins with the colonization of the stomach lining by Helicobacter pylori (H. pylori). This bacterium spreads mainly via the oral-oral route through saliva or shared utensils, and can also be transmitted in overcrowded or unhygienic environments through contaminated water, despite its brief survival outside the body.ColonizationOnce ingested, H. pylori enters the stomach and begins colonization by navigating through the mucus layer lining the stomach wall. It...