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Updated: Aug 17, 2026

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
Published on: April 1, 2019
[Cloning and sequence analysis of human uric acid transporter gene]
Quan Hong1, Di Wu, Xiang-mei Chen
1Center of Nephrology and Key Laboratory of PLA, General Hospital of the PLA, Beijing 100853, China. redhq@163.com
Objective:
To obtain full-length human urate transporter (hUAT) gene.
Methods:
Primers was designed according to the sequence of hUAT reported in Genbank. The target fragments were obtained by reverse transcriptional (RT) PCR from the mRNA extracted from human renal tubular epithelial cell lines (HK-2), followed by cloning into pEGFP-C1 plasmid. The cloned fragment was subjected to restriction mapping and sequence analysis for confirmation.
Results And Conclusion:
hUAT gene with correct sequence is successfully cloned from HK-2 cells. The restriction maps and sequence analysis of the selected clones were consistent with the sequence reported in Genbank.
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