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Updated: Aug 17, 2026
Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
[Establishment and application of real-time fluoriescene-based metry polymerase chain reaction for hepatitis B virus
Jian-kun Shen1, Rong Zhou, Zhan-hui Wang
1Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. shjk_2003@163.com
Objective:
To explore a new method for determining hepatitis B virus (HBV) genotypes B-D with real-time fluorescence-based PCR.
Methods:
The PCR primers and probes were designed according to the analysis of 143 complete HBV genomes from GenBank and on the basis of a search for genotype-specific nucleotide sequences which were conserved in the 3 HBV genotypes. Real-time fluorescence-based PCR was performed for HBV genotyping of 128 samples collected from Lanzhou. Three samples of each genotype of B, C and D were randomly selected and their S gene was sequenced for confirmation of the results of PCR-based method.
Results:
Real-time PCR identified genotype B in 26 (20.3%), genotype C in 92 (71.9%), and genotype D in 10 (7.8%) cases. The sequencing results of the S gene of 18 PCR-produced clones were in complete consistence with those of fluorescence-based PCR.
Conclusion:
The real-time PCR method is convenient, highly sensitive, rapid and accurate, especially suitable in clinical and large-scale epidemiological studies.
