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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
Published on: May 5, 2023
[c-myc gene silencing in K562 cells with RNA interference]
Feng Yue1, Wen-li Ma, Yan-bin Song
1Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China. yuefeng@fimmu.com
Objective:
To study the inhibitory effect of small interfering RNA (siRNA) targeting c-myc gene in K562 cells.
Methods:
siRNAs targeting the site 1357 of c-myc mRNA was designed and synthesized. In vitro cultured K562 cells were transfected with lipofectamine 2000 and the inhibitory effect was detected by reverse transcriptase (RT)-PCR, cell count, MTT assay and fluorescence-activated cell sorting.
Results:
Compared with the negative and blank control group, the transfection group showed marked decrease in the c-myc expression and the K562 cells exhibited increased apoptosis rate.
Conclusion:
RNA interference can effectively inhibit c-myc expression and induce apoptosis in K562 cells.
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