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Related Experiment Videos

Global gene expression profiling of circulating tumor cells.

Denis A Smirnov1, Daniel R Zweitzig, Bradley W Foulk

  • 1Immunicon Corporation, Huntingdon Valley, Pennsylvania 19006, USA.

Cancer Research
|June 17, 2005
PubMed
Summary

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Cell State and Cell Type: Deconvoluting Circulating Tumor Cell Populations in Liquid Biopsies by Multi-Omics.

Cancers·2023

Researchers developed a new method to detect circulating tumor cells (CTCs) in blood. This technique identifies CTC-specific genes, aiding in the diagnosis and management of metastatic cancers.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Metastatic tumors are a leading cause of cancer mortality.
  • Circulating tumor cells (CTCs) in peripheral blood are linked to poor outcomes in metastatic cancer patients.
  • Characterizing CTCs can improve disease management.

Purpose of the Study:

  • To develop a novel approach for identifying molecular markers to detect and characterize CTCs.
  • To generate global gene expression profiles from CTCs.
  • To identify cancer-specific and CTC-specific genes.

Main Methods:

  • Integrated platform for immunomagnetic isolation and immunofluorescent detection of CTCs.
  • RNA extraction from CTC-enriched and CTC-depleted blood fractions.
  • Global gene expression profiling of CTCs.

Related Experiment Videos

  • Quantitative real-time reverse transcription-PCR for gene expression analysis.

    • Main Results:

    • Successfully obtained blood with > or = 100 CTCs from metastatic colorectal, prostate, and breast cancer patients.
    • Generated the first global gene expression profiles from CTCs.
    • Identified a list of cancer-specific, CTC-specific genes (e.g., AGR2, S100A14, S100A16, FABP1).
    • Confirmed CTC-specific gene expression in 74 metastatic cancer patients and 50 normal donors.
    • Demonstrated that CTC gene expression profiles can distinguish cancer patients from normal donors and differentiate between cancer types.

    Conclusions:

    • A novel method for CTC detection and characterization using gene expression profiling has been established.
    • Identified specific genes useful for detecting CTCs in advanced cancer patients.
    • CTC gene expression profiles hold potential for diagnosing advanced cancer and differentiating between metastatic cancer types.