Related Experiment Video

Updated: Aug 17, 2026

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
11:48

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Published on: April 4, 2019

Interaction between the respiratory syncytial virus G glycoprotein cytoplasmic domain and the matrix protein

Reena Ghildyal1,2,3, Dongsheng Li4, Irene Peroulis1

  • 1Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Australia.

The Journal of General Virology
|June 17, 2005
PubMed

Insights

The matrix protein (M) of respiratory syncytial virus (RSV) interacts with the G glycoprotein (G) via its N-terminal residues 2-6. This interaction is crucial for viral assembly at the cell membrane.

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Paramyxovirus assembly involves matrix protein interactions with viral glycoproteins at the cell membrane.
  • Specific protein interactions during respiratory syncytial virus (RSV) assembly are not fully understood.

Purpose of the Study:

  • To investigate the interaction between the RSV matrix protein (M) and the G glycoprotein (G).
  • To identify the specific regions of G glycoprotein involved in M protein interaction.

Main Methods:

  • Co-localization studies in RSV-infected and transfected cells.
  • Expression of wild-type and mutant G glycoproteins.
  • Cell-free binding assays.

Main Results:

  • Partial co-localization of M and G proteins was observed.
  • Truncated G variants lacking the cytoplasmic domain showed reduced M interaction.
  • The N-terminal amino acids 2-6 of G, specifically serine at position 2 and aspartate at position 6, are critical for M-G interaction.

Conclusions:

  • The N-terminal region of RSV G glycoprotein is essential for its interaction with the M protein.
  • This M-G interaction likely plays a significant role in the assembly of RSV virions at the host cell membrane.

More Related Videos

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection09:01

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

Published on: December 10, 2013

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus09:08

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Published on: July 27, 2021

Related Concept Videos

Respiratory Syncytial Virus Disease01:29

Respiratory Syncytial Virus Disease

Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...
Coronavirus01:29

Coronavirus

Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...
Rab Proteins01:14

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Inhibitors Of Virion Release01:25

Inhibitors Of Virion Release

Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...
Cytomegalovirus Disease01:27

Cytomegalovirus Disease

Cytomegalovirus (CMV) disease is caused by human cytomegalovirus, a double-stranded DNA virus of the Herpesviridae family. While primary CMV infection is often asymptomatic in immunocompetent individuals, the virus can cause severe disease in neonates and immunocompromised patients. CMV is the most common cause of congenital viral infection in the United States, and a major pathogen in solid organ and hematopoietic stem cell transplant recipients.CMV is transmitted via bodily fluids, sexual...
Inhibitors of Virion Maturation and Assembly01:19

Inhibitors of Virion Maturation and Assembly

As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...