Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression in stem cell-supporting stromal cell lines.

Pierre Charbord1, Kateri Moore

  • 1Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.

Annals of the New York Academy of Sciences
|June 17, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Gene coexpression networks: concepts and applications].

Biologie aujourd'hui·2025
Same author

A multistep computational approach reveals a neuro-mesenchymal cell population in the embryonic hematopoietic stem cell niche.

Development (Cambridge, England)·2024
Same author

The EHA Research Roadmap: Hematopoietic Stem Cells and Allotransplantation.

HemaSphere·2022
Same author

Portal fibroblasts with mesenchymal stem cell features form a reservoir of proliferative myofibroblasts in liver fibrosis.

Hepatology (Baltimore, Md.)·2022
Same author

The EHA Research Roadmap: Normal Hematopoiesis.

HemaSphere·2021
Same author

In vivo screen identifies a SIK inhibitor that induces β cell proliferation through a transient UPR.

Nature metabolism·2021
Same journal

Multiomics Profiling During Autoimmune Demyelination Highlights a Complex Regulatory Role for Ataxin-1 in B Cells.

Annals of the New York Academy of Sciences·2026
Same journal

Global Trends in Light Pollution and Their Relationship With Socioeconomic Factors.

Annals of the New York Academy of Sciences·2026
Same journal

Wired for Corruption: Inter-Brain Synchrony Encodes Bribery-Related Value Information and Predicts Bribery Agreement.

Annals of the New York Academy of Sciences·2026
Same journal

LM-YOLO: A Lightweight Multi-Scale Enhanced Model for Forest Smoke Detection Using Unmanned Aerial Vehicles.

Annals of the New York Academy of Sciences·2026
Same journal

Polyrhythm Perception and Production: A Scoping Review.

Annals of the New York Academy of Sciences·2026
Same journal

DARTS-CNN-BiLSTM: Intelligent Fault Diagnosis for Computer Numerical Control Machine Tool Feed System.

Annals of the New York Academy of Sciences·2026
See all related articles

Researchers identified over 300 gene products in fetal liver stromal cells that support hematopoietic stem cells (HSCs). These HSC-supportive cells are immature and reactive, forming a dynamic niche environment crucial for stem cell regulation.

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • The stem cell niche, comprising microenvironmental stromal cells and hematopoietic stem cells (HSCs), regulates stem cell behavior.
  • The molecular composition of this niche, particularly the stromal cell milieu, is not fully understood.
  • Identifying key molecules is essential for understanding HSC self-renewal and commitment.

Purpose of the Study:

  • To define and categorize known gene products expressed by HSC-supportive stromal cell lines.
  • To compare gene expression profiles between HSC-supporting and non-supporting fetal liver stromal cell lines.
  • To gain insights into the molecular mechanisms underlying the stem cell niche's function.

Main Methods:

  • Utilized cDNA subtraction and array expression methodologies.

Related Experiment Videos

  • Curated data from the Stromal Cell Database (StroCDB) for gene products enriched in the AFT024 cell line.
  • Performed global expression analyses on multiple fetal liver stromal cell lines using microarrays.

    • Main Results:

    • Identified over 300 known gene products expressed in HSC-supporting stromal cells compared to non-supporting lines.
    • Selected gene products included transcription factors, cell membrane proteins, cytoskeleton proteins, extracellular matrix proteins, cell adhesion molecules, and cytokines.
    • HSC-supportive cells were characterized as immature, sessile, and highly reactive to integrin ligands and cytokines.

    Conclusions:

    • Fetal liver stromal cells provide a complex molecular milieu supporting HSCs.
    • HSC-supportive cells create a dynamic niche environment capable of responding to molecular cues.
    • This study surveys key proteins involved in HSC support and highlights intercellular communication within the stem cell niche.