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A semiautomated flow injection procedure for acetylcholinesterase and cholinesterase activities
I López García1, F Ruiz Abellán, M Hernández Córdoba
1Department of Analytical Chemistry, University of Murcia, Spain.
Analytical Biochemistry
|January 1, 1992
Abstract:
A stopped-flow FIA adaptation of the classical Ellman's colorimetric procedure for the measurement of acetylcholinesterase or cholinesterase activity is described. The samples are injected into a flow analytical system which is provided with an electronic timer and an electrovalve in order to stop the flow when the sample is inside the measurement cell. In this way, the absorbance-time relationship is obtained with a savings of sample, time, and reagents.