Related Experiment Video
Updated: Aug 17, 2026
A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo
Published on: August 28, 2014
[Construction of the scaffold model from heterogeneous small blood vessels]
Huayong Chen1, Shuling Bai, Jun Wang
1Department of Tissue Engineering, China Medical University, Shenyang Liaoning, 110001, PR China. chenhy6039@yahoo.com.cn
Objective:
To establish a scaffold model from heterogeneous small blood vessels.
Methods:
Caudal arteries from 34 Wistar rats (average length 12.08 +/- 1.69 cm) were made into acellular blood vessel scaffolds. Some scaffolds were observed by electron microscope, and others were transplanted to the cut ends of ear central arteries of male Japanese big ear white rabbits.
Results:
Average external diameter was 0.74 +/- 0.08 mm in proximal, and 0.55 +/- 0.08 mm in distal end of rat caudal arteries. The small blood vessel scaffolds had shin wall which was white and soft, composed of fibrous tissues without cells. On the intima surface the fibrous tissues were arrayed densely in a grid-like pattern. After transplantation, the blood flow was reserved, and kept flowing freely in 24 hours. The pulsation of the transplanted artery was accessible and no blood leakage was found.
Conclusion:
The natural scaffolds are composed of fibrous tissues, and can sustain the artery pulse pressure for 24 hours. It is better to suture the blood vessels by sleeve anastomosis.
