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Published on: January 7, 2019
Expression of CD44v6 gene in normal human peripheral blood
Jian Song1, Dong-Sheng Zhang, Jie Zheng
1Department of Pathology, Medical School of Southeast University, Nanjing 210009, Jiangsu Province, China.
Aim:
To investigate if CD44v6 could be used as a molecular marker of cancer progression and metastasis through the detection of CD44v6 gene expression in normal human peripheral blood.
Methods:
RNA was extracted from the peripheral blood mononuclear cells of 50 healthy donors, the expression of CD44v6 was investigated using reverse transcriptase-polymerase chain reaction (RT-PCR).
Results:
CD44v6 mRNA was detected in 58% of healthy volunteers under the proper controls.
Conclusion:
Our results suggest that the measurement of CD44v6 expression in peripheral blood by RT-PCR is not suitable for detection of circulating tumor cells.
