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Seladin-1/DHCR24 expression in normal ovary, ovarian epithelial and granulosa tumours
Peter J Fuller1, Maria Alexiadis, Tom Jobling
1Prince Henry's Institute of Medical Research, Department of Gynaecology Oncology, Monash Medical Centre, Clayton, Victoria 3168, Australia. peter.fuller@phimr.monash.edu.au
Objective:
The human DIMINUTO/DWARF1 homolog seladin-1/DHCR24 has been recently reported to be up-regulated in adrenocortical adenomas. Seladin-1 expression has been reported in the normal ovary. Granulosa cell tumours of the ovary (GCT) as with adrenocortical adenomas arise from a steroidogenic tissue, respond to pituitary hormone stimulation and synthesize steroid hormones.
Design:
To test the hypothesis that seladin-1 may also have a role in the pathogenesis of GCT, we determined the expression of seladin-1 in a cohort of GCT and in mucinous and serous cystadenocarcinomas and in normal ovary.
Measurements:
Expression was determined by RT-PCR using gene specific primers and probes combined with Southern blot analysis of the PCR products.
Results:
Seladin-1 expression was identified in the normal ovary, mucinous cystadenocarcinomas and serous cystadenocarcinomas of the ovary whereas no expression was observed in the GCT.
Conclusions:
Based on our results, seladin-1 is not expressed in the granulosa cells or at least not in those that give rise to GCT.
