Direct interaction between SNAP-23 and L-type Ca2+ channel
Won Jin Cho1, Aleksandar Jeremic, Bhanu P Jena
1Department of Physiology, Wayne State University School of Medicine, Detroit, MI, 48201, USA.
Abstract:
During secretion, membrane-bound secretory vesicles dock and fuse at the base of porosomes in the cell plasma membrane. Among other proteins, the porosome is composed of SNAREs and Ca2+-channels. Ca2+-channels and SNAREs have been implicated in cell secretion. Several immunoprecipitation and binding studies suggest the physical interaction of the t-SNARE proteins, Syntaxin-1 and SNAP-25 with various Ca2+-channels. In this study, using yeast two-hybrid and immunoanalysis, we demonstrate for the first time, direct interaction of SNAP-23 and a L-type Ca2+-channel at the plasma membrane in pancreas.
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