Transcervical artificial insemination in the cat
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Updated: Aug 17, 2026

Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination
Published on: July 7, 2023
Daniele Zambelli1, Marco Cunto
1Veterinary Clinical Department, Obstetric-Gynecological Section, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy. zambelli@vet.unibo.it
Abstract:
Many studies have reported new reproductive techniques for reproduction in endangered non-domestic felids. Artificial insemination is an important tool for developing breeding programs. This manuscript reviews recent progress in feline artificial insemination, with particular emphasis on intrauterine sperm deposition. Intrauterine insemination of felids results in higher fertilization rates than intravaginal insemination, despite insemination with fewer sperm. Although surgical insemination was utilized historically, there are some reports of catheterization of the cervix of the queen. Recently, a new technique and catheter were proposed for transcervical insemination of fresh or frozen-thawed semen.

